Development of Milk-based Thabdi by using Peanut as an Ingredient

Background: Thabdi is a milk-based sweet delicacy, produced and consumed widely in the Saurashtra region of Gujarat State. It is characterized with brown colour, granular texture and caramelized flavour. Peanuts being globally popular oil seed crop is considered as good source of fat, protein, carbohydrates, essential amino acids, minerals and other growth-promoting elements. In present study, peanut was included as ingredient for development of milk-based peanut thabdi. Methods: Brief method of milk-based peanut thabdi preparation included heat desiccation of milk till pre-pat formation stage, further heating with clarified milk fat ( ghee ) to get desired granular texture, followed by heating with added sugar and roasted peanut powder till desired brown colour is obtained. Fat/solid-not-fat (SNF) ratio of milk, peanut and sugar levels were optimized using response surface methodology. Result: The fat/SNF ratio of milk 1.11, 21% heat-treated and ground peanut (% w/w of milk solids, dry basis) and 80% sugar (% w/ w of milk solids, dry basis) level were found to be optimum with a desirability of 0.92. Optimized thabdi samples indicated better acceptability with overall acceptability score of 8.54±0.03 compared to market samples. The product remained acceptable on 21 st day with overall acceptability score of 7.21±0.10, when packed and stored at 7±2  C.


INTRODUCTION
In India, traditional milk products are estimated to account for nearly half of milk produced and their market is growing rapidly (Bandyopadhyay et al., 2006).Traditional dairy products are characterized with a distinct flavour, texture and appearance, which are important to consumers in terms of cultural, social and religious reasons (Pal and Raju, 2010).Thabdi is a traditional heat-desiccated milk product that is particularly popular in Gujarat, having granular texture and caramelized flavour.Thabdi has potential to popularize in other parts of the country, as food processors are looking to diversify product range and hence attracted interest of researchers.Characterization study for thabdi was conducted by Patel et al. (2012), who observed wide range of quality characteristics of thabdi samples being sold in market.Process standardization for preparation of thabdi or thabdi peda was attempted by Hirpara et al. (2015), Krupa et al. (2013) and Modha et al. (2015).Hirpara et al. (2020) later attempted to incorporate ghee residue (a byproduct of clarified milk fat manufacture) in thabdi preparation.
Peanuts are a globally significant oilseed crop that is a good source of protein, oil, fiber and various healthpromoting elements.Peanuts are a good source of fat, protein, carbohydrates, essential amino acids, minerals and other growth-promoting elements.The phytonutrient content of peanuts, which includes resveratrol, phenolic acids, isoflavonoids and phytosterols, has also recently been reported to play significant role in human health and wellbeing (Toomer, 2018).Peanuts, if consumed daily, can aid in prevention of malnutrition (Pelto and Armar-Klemesu, 2011).Food researchers look into of options for creating new valueadded products with enhanced nutritional properties without compromising with sensory quality in order to meet consumer expectations.The peanut powder supplemented burfi was optimized by Yadav et al. (2018).Sejani et al. (2022) studied the effect of defatted peanut flour on the composition of the thabdi peda.The present study was undertaken to design a technique for the manufacture of milk-based thabdi using peanuts as an ingredient.

MATERIALS AND METHODS
The research work carried out at College of Dairy Science, Amreli, Gujarat, India during 2019-2021. Milk, peanuts (Variety-Gujarat 20) and sugar were purchased from the local markets of Amreli district.The average length and width (n = 50) of grains were the grain were 14.48±0.32mm and 8.21±0.12mm, respectively.For processing of peanuts, heating 800 g of sand was heated in stainless steel karahi (round bottom shallow pan) till temperature reached 105±2C in.Raw peanuts (300 g) were added to sand and heated at 105±2C for 4 min with continuous stirring.Heattreated peanuts were separated from sand and ground using domestic mixer-grinder after removal of testa and germ.Thabdi was prepared adopting the procedure described by Hirpara et al. (2015) with modifications.Fat/SNF ratio of milk was adjusted as per formulation indicated in experimental design matrix (Table 1).Milk was subjected to boiling in open pan and desiccation continued till pre-pat formation (2 fold concentration) stage.The mass was maintained for 10 min.Desiccation continued with the addition of ghee at a rate of 1.2% (% w/w milk basis) till grains are observed.Ground sugar and peanut powder were added as per experimental design matrix (Table 1) and desiccation was continued until desired colour is obtained.The product was then emptied in stainless steel tray and spread to the desired thickness and cut into cuboid shapes.
Response surface methodology tool of Design-Expert (V-10) software was used to study the effect of fat/SNF ratio, peanut and sugar level on the sensory quality of milk-based peanut thabdi and their levels were optimized using numerical optimization technique.Lower and higher levels for fat/SNF ratio, peanut and sugar level were 0.6 and 1.5, 21 and 80 (%w/w milk solids, dry basis) and 45 and 80% (%w/w milk solids, dry basis), respectively for design of experiments.Experimental design matrix as suggested by Design-Expert (V-10) is presented in Table 1.The optimized formulation of milk-based peanut thabdi obtained as a result of numerical optimization was prepared, vacuum packed in 75  LDPE pouch and stored at 7±2C.Packed samples were evaluated at regular intervals of 0, 7, 14, 21 and 28 days for changes in p hysico chemic al, microbiological and sensory qualities.The moisture, fat (Mojonnier method), protein (Microkjeldahl method), ash and acidity of milk-based peanut thabdi was estimated by adopting procedure mentioned in SP: 18 Part XI (1981).The pH was measured using a pH meter (Oakton, Model PC 2700) by dissolving 1 g of sample in 10 ml warm distilled water.The method outlined by Thomas et al. (1954) was used to determine the free fatty acids (FFA).Using a water activity meter (Novasina, Switzerland), the water activity (aw) of milk-based peanut thabdi samples was measured at a temperature of 25C.Microbiological examination such as standard plate count, coliform count and yeast and mould count was enumerated using SP-18 Part XI (1981) procedures.Texture profile analysis of milk-based peanut thabdi and market samples of thabdi was carried out using a TA-XT plus Texture Analyzer (Stable Microsystem).Compression was applied to the samples using a P/25 cylindrical probe and characteristics such as hardness, cohesiveness, springiness, resilience and gumminess were measured.The pre-test and test speeds were maintained at 1.0 mm/s.Post-test speed remained constant at 5.00 mm/sec.The probe was allowed to travel in the samples to a depth of 8.00 mm.Sensorial assessment of the milkbased peanut thabdi was performed using 9 points hedonic scale (9=like extremely; 1=dislike extremely).Sensory evaluation was performed by eight panelists and tested for colour and appearance, body and texture, flavour and overall acceptability.For optimization, the mean scores of the eight panelists for specific attributes were considered as responses.Design-Expert software was used to analyse the data obtained during optimization trials.Significant differences between mean values were assessed by analysis of variance (ANOVA) tests at p-value of 0.05.

RESULTS AND DISCUSSION
Milk-based peanut thabdi was prepared using different formulation, as suggested by the Design-Expert software and effect of Fat/SNF ratio, peanut and sugar levels on sensory quality is presented in Table 1.Regression equation to predict the sensory responses are presented in Table 2.

Colour and appearance
The regression analysis of the colour and appearance score presented in Table 3 reveals that the coefficient of determination (R 2 ) for the quadratic model of colour and appearance was 0.86.The adequate precision value was found to be 10.60 appreciably higher than the minimum desirable 4 (for high prediction ability), indicating that this model was highly significant (p<0.01).The effect of peanut and sugar levels were found to be significant on colour and appearance at p<0.05 and p<0.01 level of significance, respectively.The effect of the sugar and peanut level on the colour and appearance score of milk-based peanut thabdi is shown in Fig 1a .An increase in the level of peanut initially leads to an increase in the colour and appearance score, but at higher levels, the colour and appearance score decreased.The sugar level had major influence on the development of desired colour and appearance of milk-based peanut thabdi.Increase in sugar content lead to increase in colour and appearance score of milk-based peanut thabdi (Fig 1a).Krupa et al. (2013) noticed a constant increase in the colour and appearance score of thabdi when the amount of sugar added to thabdi was increased from 6% to 10% and also observed that extended heating period may indicated detrimental effect on the colour and appearance of thabdi.Increase in 5-hydroxymethyl furfural (HMF) concentration was reported by previous workers with increase in sugar levels, probably as a result of maillard browning and caramelization occurring during production.

Body and texture
The body and texture scores of milk-based peanut thabdi ranged from 7.00 to 8.83.The coefficient of determination (R 2 ) for the quadratic model was 0.72.The regression analysis of the data presented in Table 3 reveals adequate precision value of 9.82.The effect of sugar level on the body and texture scores was highly significant (p<0.01).Modha et al. (2015) reported that increased sugar and fat levels improved body and texture scores on a linear level, resulting in a soft body and smooth granules in thabdi peda.A similar trend was observed in our study for the milk-based peanut thabdi.Minimum and maximum scores for body and texture

Flavour
Flavour is undoubtedly the most essential criterion for determining acceptance of a new product to consumers.
The flavour score of milk-based peanut thabdi ranged from 7.33 to 8.83 .Th e regression analysis of the d ata presented in Table 3 revealed that the coefficient of determination (R 2 ) for the quadratic model was 0.74.Furthermore, the statistical analysis indicated that the mod el fitted the observed data well with adequ ate precision value of 9.14 and model was highly significant (p<0.01).Peanut and sugar levels significantly (p<0.01)affected the flavour score of milk-based peanut thabdi.
Maxim um flavo ur sc ore of 8 .83was fou nd fo r the experiment number 8 in Table 1.Modha et al. (2015) reported during study on thabdi peda noted that owing to enhanced sweetness and caramelized flavour, sugar has a linear positive influence on the flavour score of thabdi peda.

Overall acceptability
The coefficient of determination (R 2 ) for the quadratic model was 0.75 (Table 3).The adequate precision value was found to be 9.06 which was appreciably higher.The minimum (7.17) and maximum (8.80) overall acceptability score was obtained for the formulation indicated in experiment number 19 and 1, respectively.The effect of peanut and sugar levels on the overall acceptability score of milk-based peanut thabdi were highly significant (p<0.01).The effect of peanut and sugar levels on the overall acceptability score of milkbased peanut thabdi is shown in Fig 1d .Similar trends were also for thabdi peda samples by Modha et al. (2015), who observed that positive influence of milk fat and sugar level on the overall acceptability of thabdi peda.

Optimization of milk-based peanut thabdi
Optimization was aimed at achieving the best levels of factors such as Fat/SNF ratio of milk, peanut and sugar levels based on maximizing reponses obtained for sensory scores.A Fat/SNF ratio of milk 1.11, 21% heat-treated and ground peanut level (% w/w of milk solids, dry basis) and 80% sugar level (% w/w of milk solids, dry basis) were found to be optimum as a result of numerical optimization with a desirability of 0.920.The optimized milk-based peanut thabdi had a total solids content of 82.58±0.2%,moisture content of 17.42±0.2%,fat content of 28.5±0.36%,protein content

Compositional aspects
The comparison between the optimized formulations with two market samples (A and B) in terms of compositional parameters is given in Table 4. Market sample B contained the highest total solids (83.51 ±0.09%), ash (2.31±0.08%)and carbohydrates (49.5±0.47%),whereas market sample A contained the highest moisture (20.18±0.11%)and protein (13.5±0.28%).The optimized formulations had a higher fat level (28.5±0.36%)than the market samples (A and B), which might be due to use of peanut ingredient (Table 4).

Textural parameters
The texture of the popular market thabdi has firm to slightly loose grains with small pools of liquid fat or solid fat on the surface.Table 4 shows the comparison between the optimized formulations with two market samples (A and B)

Sensory characteristics
The popular market thabdi had brown to dark brown c olou r, ric h n utty, co ok ed, g hee-like flavou r an d characteristic granular texture.The comparison between the optimized formulations with two market samples (A and B) for sensory quality is presented in

Effect of refrigerated storage on the physicochemical, sensory and microbial quality of milk-based peanut thabdi
Optimized milk-based peanut thabdi was prepared, vacuum packed in a 75  LDPE pouch and stored in a refrigerator at 7±2C for 28 days.The data pertaining to changes in the physicochemical, sensory and microbial qualities of milkbased peanut thabdi are given in Table 5.The initial moisture content of the milk-based peanut thabdi was 17.42±0.202%.There was no significant decrease in moisture content at the end of 28 days of storage under refrigeration (Table 5).However, the water activity of the milk-based thabdi samples decreased significantly during storage.The findings for water activity by Londhe et al. (2012) also indicated that storing brown peda 30C causes decrease water activity due to loss of moisture during storage.The initial titratable acidity (% lactic acid) of milk-based peanut thabdi was 0.315 which increased to 0.387 after 28 days of storage (Table 5).A significant (p<0.05)increase in acidity was observed during refrigerated storage of the samples after 28 days of storage.At 30C the acidity of brown peda were reported to increase considerably in various techniques of packaging (Londhe et al., 2012).According to Sharma et al. (2003), the acidity of malai peda increased during storage when packed in Poster Paper/Al-foil/LDPE.Sachdeva and Rajorhia (1982) also noted increased in acidity during the storage of burfi.The pH of the samples significantly (p<0.05)decreased after 7, 14 and 28 days of storage.Kumar et al. (1997) noted that the pH of peda dropped while during storage at a temperature of 20C.
The free fatty acids (FFA) content of fresh milk-based peanut thabdi samples was 0.85 (% oleic acid), which increased significantly (p<0.05) after 21 days of storage at 7±1C (Table 5).Comparable rise in free fatty acid concentration in brown peda samples during storage was also reported by Londhe et al. (2012).Increase in FFA of milk-based peanut thabdi samples could be attributed to the metabolic activity of microorganisms during storage (Samaržija et al., 2012).There was a significant (p<0.05)increase in the hardness (g) of the milk-based peanut thabdi samples from 2706 to 3080 during 28 days of storage under refrigeration, which could be attributed to the loss of moisture during storage which depends on the type of package and storage conditions.
Enumeration of standard plate count (SPC) and yeast and mould (YandM) count of milk-based peanut thabdi samples revealed a significant increase from the initial count of 3.57 and 1.23 log cfu/g to 4.37 and 3.97 log cfu/g, (Table Development of Milk-based Thabdi by using Peanut as an Ingredient 5), respectively when stored under refrigeration for 28 days.Coliform count was not detected when the first dilution was plated on violet red bile agar (VRBA).Storage study of burfi at 30C and 5C by Sachdeva and Rajorhia (1982) reported increase in SPC and YandM count.The SPC as well as YandM during storage of brown peda was observed (Londhe et al., 2012).Rise in SPC during the storage of burfi was also observed by many researchers (Garg and Mandokhot, 1987;Misra and Kuila, 1988).
Changes in the sensory quality of milk-based peanut thabdi samples were significant when packed samples were stored at 7±2C for 28 days (Table 5).The colour and appearance score of milk-based peanut thabdi significantly (p<0.05)decreased after 21 days of storage.The body and texture scores of milk-based peanut thabdi significantly (p<0.05)decreased after 14 days of storage.The flavour score of milk-based peanut thabdi significantly (p<0.05)decreased after 14 days of storage.The overall acceptability score of milk-based peanut thabdi was significantly (p<0.05)reduced from 8.47 7.43 at the end of 21 days of storage, which further reduced to 6.93.Hence, milk-based peanut thabdi can be acceptably stored in a 75  LDPE pouch at refrigerated temperature (7±2C) for 21 days.Shelf life of malai peda as affected by modified atmosphere packaging (MAP) was examined by Sharma et al. (2003).Author reported that malai peda packed in Poster Paper/Al-foil/ LDPE and stored at 11C and 52% RH, the product was acceptable for up to 31 days.Packaging techniques such as vacuum packaging and MAP were reported to enhance the shelf life of brown peda samples when compared to conventional packaging (Londhe et al. 2012).

CONCLUSION
Peanuts were successfully incorporated as an ingredient in the preparation of milk-based peanut thabdi.Heat treatment of peanuts at 105±2C for 4 min in sand improved the sensory quality of the product.Thabdi prepared using milk of 1.11 Fat/SNF ratio, 21% heat-treated and grinded peanut (% w/w of milk solids, dry basis) and 80% sugar (% w/w of milk solids, dry basis) were found to be optimum based on sensory scores, which remained stable up to 21 days when vacuum packed and stored at 7±2C.
Fig 1c also revealed that as sugar levels increased, th e flavour score o f the milk-b ased peanut th ab di formulation also increased.

Fig 1 :
Fig 1: Response surface plot of sensory attributes: Colour and appearance score (a); Body and texture score (b); Flavor score (c); and overall acceptability score (d); as influenced by peanut level and sugar level.

Table 2 :
Regression equation for prediction of sensory quality of milk-based peanut thabdi.

Table 3 :
ANOVA of fat/SNF ratio, peanut and sugar levels on the sensory quality of milk-based peanut thabdi.

Table 1 :
Effect of fat/SNF ratio, peanut and sugar levels on the sensory attributes of milk based peanut thabdi.Development of Milk-based Thabdi by using Peanut as an Ingredientscore were obtained for experiment number 17 and 18, respectively (Table1).Increasing sugar levels increased the body and texture scores of milk-based peanut thabdi Yadav et al. (2018)-based Thabdi by using Peanut as an Ingredient of 8.4±0.26%,ashcontent of 2.52±0.05%andcarbohydratecontent of 43.16±0.37%(Table4).Optimized formulation of peanut powder-supplemented burfi suggested byYadav et al. (2018)contained 10% peanut powder, 80% khoa and 20% sugar.

Table 4 :
Comparison of milk-based peanut thabdi with market samples in terms of compositional, textural and sensory quality.bValues in each cell represent mean±SE, (n=3, 5, 8 for compositional, textural and sensory attributes, respectively).Means with different superscript in a row vary significantly (p<0.05).

Table 5 :
Physico-chemical, sensory and microbial changes of milk-based peanut thabdi during refrigerated storage.

Table 4 .
The sensory attributes of the optimized formulation of milkbased peanut thabdi, viz.colour and appearance, body and texture, flavour and overall acceptability, were significantly different (p<0.05) from those of market sam ple B. Ho wever, th e op tim ized p ro d uc ts w ere comparable to those of market sample A.