Appraisal of Salt Sensitivity among Elite Genotypes of Chickpea ( Cicer arietinum L.) At Seedling Stage

genotypes identified as the source of salinity tolerance were HC5, HC3, HC1, H12-22, H14-14


INTRODUCTION
The cool-season grain legume chickpea (Cicer arietinum L.) crop, a self-pollinated diploid plant (2n= 2x= 16) ranked third after common bean and field pea (Janghel et al., 2021), is vulnerable to salt stress than other crops (Flowers et al., 2015;Kumar et al., 2018).Soil salinity is an ever-increasing threat around the world with the use of saline water for irrigation under current climatic conditions (Dias et al., 2016).Now, the time has come up to think about it seriously with an alarming rate of transforming agricultural land into saline soil for various reasons including low precipitation, high evaporation rate and high solar balance.Chickpea is typically farmed in arid and semi-arid regions of the world, where soil salinization is a problem (Flowers et al., 2010).India now leads the globe in both area and production of chickpeas, accounting for 70.57percent of the worldwide area and 69.21 percent of global production followed by Australia (Sharma et al., 2020).It is an essential component of crop rotations (Zawude and Shanko, 2017), improves soil health by fixing atmospheric nitrogen through a symbiotic relationship with Rhizobia, and is gradually becoming accepted as a future staple food crop due to its high nutritional content, market value, and adaptability (Kaashyap et al., 2017).
Especially salinity in chickpea, NaCl salt inhibits the rate and extent of seed germination, as well as plant establishment (Flowers et al., 2010).Plant growth is hampered by salts due to an increase in soil osmotic pressure and interference with plant nourishment (Machado and Serralheiro, 2017).Salt stress slows seed imbibition, radicle elongation, seed germination, and seedling growth due to osmotic effects (Kumar et al., 2018;Pereira et al., 2019).The high salt concentration in the soil solution limits the plant's ability to absorb water (Acosta-Motos et al., 2017), carbon dioxide absorption by promoting stomata closure (Osakabe et al., 2014), and the photosynthetic ability of dry matter accumulation (Silva et al., 2019).Chickpea germplasm has been tested for salt sensitivity and genetic diversity in seed production and component properties (Pushpavalli et al., 2020), but more extensive research work at the seedling stage is required.Thus, it is a pelion to manage the saline soil, so there is a sturdy need to screen the genotypes and develop the salt tolerant cultivars, which is the simplest and most cost-effective strategy to manage the soil salinity stress.The purpose of this study was to determine the salt sensitivity of chickpea genotypes at the seedling stage under varying levels of salt stress based on seedling growth, vigor, and physiological performance.

MATERIALS AND METHODS
The 20 Desi chickpea genotypes shown in Table 1 were investigated for salt sensitivity during Rabi 2020-21 in the growth chamber of the Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar (Haryana), India.The seedling growth, vigor, and physiological performance were examined in a completely randomized design (CRD) with two factors (genotypes and salinity levels) in three replications at varied levels of NaCl salt (0, 4, 6, 8, and 10 dS m -1 ).The homogeneous and healthy seeds of chickpea genotype were surface sterilized with a 1% sodium hypochlorite solution.Then, the 50 seeds (10 seeds at each level) of each genotype were allowed to sprout in the germination paper dipped with desired salinity levels of NaCl salt using the between paper (BP) method of seed germination under normal room temperature conditions (Temp 25±3C and 60-70% RH).The following observations were recorded at 15 and 30 DAS (days after sowing) are as follows:

Seedling growth
Length (cm) of coleoptile and radicle measured in centimetre scale, whereas, fresh weight (FW) weighed in gram using digital balance immediately after separation of coleoptile and radicle.

Relative water content % (RWC %)
RW C % of coleoptile and radicle of chickpea seedling estimated as per the method suggested by Weatherley (1950) and calculated by using the formula: W here, FW = Fresh weight (g).DW = Dry weight (g).TW = Turgid weight (g).

Relative stress injury % (RSI %)
RSI % of coleoptile and radicle of chickpea seedling measured as per the method suggested by Sullivan and Ross (1979) and calculated by the formula: W here, ECa= Electrical conductivity before heat treatment.ECb= Electrical conductivity after heat treatment.

Statistical analysis
To assess the significant difference between the three treatments, the data was statistically analysed for ANOVA (analysis of variance) at 5% level of significance using OPSTAT online software (http://14.139.232.166/opstat/).

RESULTS AND DISCUSSION
For seedling growth, vigour, and physiological performance under varied salt levels (0, 4, 6, 8, and 10 dS m -1 ), the two factors ANOVA were shown to be substantially different at the 5% level of significance.

Relative water content (%) and relative stress injury (%)
The chickpea genotypes exhibited a similar declining pattern in the relative water content % (RWC) with the augmentation of salt stress.RWC in coleoptile varied from 48.97 to 77.11% after 15 days of sowing and from 45.39 to 74.91% after 30 days of sowing at 10 dS m-1 of salt stress (Fig 4A).Among the chickpea genotypes, H HC 5 (23.76),HC 3 (26.58),ICCV 4958 (24.93), had comparatively more RWC along with a smaller reduction in RW C at 10 dS m-1 as compared to control value at 30 DAS (Fig 4B).
Excess salts in the soil concentrated around the root zone, causing a change in cell metabolism that resulted in a decrease in RW C and an increase in RSI due to detrimental effects on physiological processes like plant water status, ion homeostasis, and photosynthesis (Babar et al., 2014).In chickpea genotypes, the RW C and RSI could be used as direct measures of salt stress resistance.Based on these physiological parameters, the salt stress tolerant  genotypes viz., H 03-56, HC 5, HC 3, HC 1, H 12-55, H 12-22, H 13-01, H 14-21, H 14-14, H 14-01, ICCV 4958 and H 15-25 had been screened and identified.These results are also in concomitant with Dudhe and Kumar (2016), Gaurav et al. (2016), Nasiri et al. (2021) and Kaur et al. (2021).

CONCLUSION
Salt stress conditions cause a delay in seed germination which led to greater exposure of seeds to pathogenic action, insect-pest attack and substantial loss in seedling vigor.It has the potential to hinder cell division and elongation, as well as limit water availability and photosynthesis, resulting in a reduction of plant growth.Seedling biomass production, growth, and development processes are all sensitive to salt stress, hence these could be employed as good selection criteria for determining salt stress tolerance in chickpea genotypes.Based on seedling growth and physiological performance under laboratory conditions, the chickpea genotypes HC 5, HC 3, HC 1, H 12-55, H 14-14, H 14-01 and H 15-25 performed relatively better than others at 10 dS m-1 salt stress and could be used as a source of tolerance in breeding programmes to develop salt-tolerant chickpea genotypes.

Table 1 :
List of 20 Desi chickpea genotypes for salt sensitivity appraisal at seedling stage.